LED Box

LED Box is an ideal irradiation device for large samples used in Biophotonics, Photobiology, Photochemistry and Photomedicine research.

The optical and mechanical parts are specially designed for precise irradiation of cellular cultures and small animals used in pre-clinical studies. Our unique design allows your sample to be easily irradiated from bottom-to-top or top-to-bottom. Hence, LED Box is a versatile device to be used in a wide range of light- mediated experiments.

LED Box is available at more than 30 peak wavelengths in the UV-VIS-NIR spectra (265–830nm). A large area (163 cm2) and homogeneous (>90%) light beam allows simultaneous irradiation of multiple samples. For quality control, each LED Box is individually calibrated by a certified spectrometer.

Dedicated software and hardware guarantees better experimental safety and reproducibility. Therefore, you can rely on robust equipment protected against overheating and burned diodes (life spam over 1,000 hours).

 

Light homogeneity (Petri factor)

According to the protocol developed by Bolton and Linden (2003), a Petri Factor is defined as the ratio of the average of the incident irradiance over the area of the Petri dish to the irradiance at the center of the dish. The same protocol can be applied to other geometries, such as in rectangular multi-well cell culture plates. This method allows a user to more accurately determine the average fluence rate of the light beam.

A Petri Factor greater than 0.9 helps to ensure all the area under a light beam is treated with more than 90% of homogeneity. This can be calculated by measuring the irradiance with a radiometer and sensor every 5 mm over the area of the collimating tube.

At BioLambda, each LED Box is tested to ensure an even intensity distribution at Petri factor levels >0.9. Therefore, we can ensure appropriate precision levels for your experiments.

 

Frequent LED Box applications

Biomedical applications:

  • Photobiomodulation (PBM)

  • Antimicrobial and Oncologic Photodynamic Therapy (PDT)

  • Microbial inactivation induced by UV or Blue Light

  • Light induced mutagenesis and carcinogenesis

  • Immune system modulation

  • Skin pigmentation

  • In vivo photosynthesis

  • Tissue optics

  • Optogenetics

    Photochemistry applications:

  • Sunscreen development

  • Drug discovery

  • Molecular synthesis

  • Molecular analysis

  • Photopolymerization

  • Nanotechnology

  • Photochemical and photothermal reactions

  • In vitro photosynthesis

  • Photocatalysis

  • Fluorescence probing

    Physics and Engineering applications:

  • Study of excited state molecules

  • Photovoltaic and photothermovoltaic

  • Quantum dots development

  • Down- and Up-converters development

  • Laser pumping

  • Light emitting systems development

  • Multispectral imaging

  • Illumination

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